Creative Biogene offers high quality, fast turnaround and cost-effective Stable Cell Line Generation Service for protein expression, gene knockdown or genome editing that meet your specific research needs. Based on many years' experience on cell line development, Creative Biogene's cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements.

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing.

Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA (~150 kb) virus in the family Herpesviridae. There are two HSV species: Herpes Simplex Virus Type I (HSV-1) and Herpes Simplex Virus Type II (HSV-2), both of which can infect humans and cause diseases.

Retroviral vectors are engineered to transfer and integrate target genetic material into the genomes of host cells. Retroviruses have several distinct advantages over other vectors, especially when permanent gene transfer is the preferred outcome. Some disadvantages of retroviruses are that they can only transduce dividing cells, and that they integrate somewhat arbitrarily into genome which has the potential to lead to oncogenesis.

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

According to the upstream regulator of the reporter gene, reporter cell lines can be further divided into two groups: 1) Constitutive Reporter Cell Lines in which reporters are constitutively expressed under the control of a constitutive, strong promoter such as CMV promoter; and 2) Inducible Reporter Cell Lines in which reporters are inducibly expressed under the control of a minimal promoter fused to multiple inducer response elements.

Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library, subtractive cDNA library, normalized cDNA library, full length cDNA library, yeast two-hybrid cDNA library and SSH cDNA library. Creative Biogene’s goal is to provide you with the most affordable and high-quality cDNA libraries construction service to ensure your satisfaction in a timely and professional manner.

Stable cell lines with specific gene over-expression or knock-down are very helpful in gene function analysis, target discovery, target validation, assay development, and compound screening. However, generation of stable cell lines is a time-consuming and expensive process.

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. The engineered CRISPR/Cas9 technology consists of a single guide RNA (sgRNA) and a human codon-optimized Cas9 nuclease.

Recombinant viruses are versatile and highly efficient tools commonly used by molecular biologist for gene delivery and expression in cells. During a period of development, Creative Biogene has already possessed sophisticated equipments, advanced technologies and highly experienced staffs which are available to provide you with GMP grade recombinant viruses.

Stable cell lines with specific gene over-expression or knock-down are very helpful in gene function analysis, target discovery, target validation, assay development, and compound screening. However, generation of stable cell lines is a time-consuming and expensive process.

Protein phosphorylation is vital for normal cellular function and a major control mechanism of a wide range of physiological processes. Phosphate groups are post-translational modifications that regulate many disease states, including cancers, neurological diseases, diabetes, pain, autoimmune disorders, and cardiovascular diseases.

Mutagenesis in the laboratory is an important molecular biology technique that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products. It is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, or for protein engineering. With years of experience in mutagenesis field, Creative Biogene can offer the most affordable and high-quality gene mutagenesis service to meet your various needs.

miRNAs play important roles in various cellular processes including apoptosis, cell division, and proliferation and others. Investigations of miRNAs will help to decipher protein functions and even discover new drugs. Creative Biogene is committed to providing outstanding miRNA products and comprehensive services to facilitate and simplify miRNA investigation.

Ubiquitin modifications can be reversed by the reaction of enzymes collectively known as deubiquitinating enzymes (DUBs). Deubiquitination is performed by the action of DUBs, ubiquitin specific proteases that can reverse target-protein ubiquitination by editing or disassembling polyubiquitin chains. DUBs play an important role in several aspects of the ubiquitin-proteasome system, and mutations in genes expressing DUBs have been implicated in a number of diseases, including hereditary cancer and neurodegeneration.

Transporters are a kind of membrane proteins controlling uptake and efflux of essential nutrients ions, neurotransmitters, cellular waste, environmental toxins, and drugs, into and out of cells and organelles. Therefore, transporters play a significant important role in drug's clinical efficacy and safety. Currently, transporters are used as therapeutic targets to treat numerous diseases such as diabetes, asthma, cardiovascular diseases, major depression, neuroscience, hypertension and constipation.

Adeno-associated virus (AAV) has been widely used by scientists to deliver exogenous genetic material into cultured cells or live animal models. AAV can infect both dividing and non-dividing cells. Multiple AAV serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ) enable AAVs to efficiently infect with broad specificity.

Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications in mammalian cells. There is no question that the adenovirus is the most effective means of delivering genes in vivo and in vitro. However construction of adenovirus vectors and virus packaging is time-consuming and labor intensive.