Stable cell lines with specific gene over-expression or knock-down are very helpful in gene function analysis, target discovery, target validation, assay development, and compound screening. However, generation of stable cell lines is a time-consuming and expensive process.

Protein phosphorylation is vital for normal cellular function and a major control mechanism of a wide range of physiological processes. Phosphate groups are post-translational modifications that regulate many disease states, including cancers, neurological diseases, diabetes, pain, autoimmune disorders, and cardiovascular diseases.

Mutagenesis in the laboratory is an important molecular biology technique that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products. It is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, or for protein engineering. With years of experience in mutagenesis field, Creative Biogene can offer the most affordable and high-quality gene mutagenesis service to meet your various needs.

miRNAs play important roles in various cellular processes including apoptosis, cell division, and proliferation and others. Investigations of miRNAs will help to decipher protein functions and even discover new drugs. Creative Biogene is committed to providing outstanding miRNA products and comprehensive services to facilitate and simplify miRNA investigation.

Ubiquitin modifications can be reversed by the reaction of enzymes collectively known as deubiquitinating enzymes (DUBs). Deubiquitination is performed by the action of DUBs, ubiquitin specific proteases that can reverse target-protein ubiquitination by editing or disassembling polyubiquitin chains. DUBs play an important role in several aspects of the ubiquitin-proteasome system, and mutations in genes expressing DUBs have been implicated in a number of diseases, including hereditary cancer and neurodegeneration.

Transporters are a kind of membrane proteins controlling uptake and efflux of essential nutrients ions, neurotransmitters, cellular waste, environmental toxins, and drugs, into and out of cells and organelles. Therefore, transporters play a significant important role in drug's clinical efficacy and safety. Currently, transporters are used as therapeutic targets to treat numerous diseases such as diabetes, asthma, cardiovascular diseases, major depression, neuroscience, hypertension and constipation.

Adeno-associated virus (AAV) has been widely used by scientists to deliver exogenous genetic material into cultured cells or live animal models. AAV can infect both dividing and non-dividing cells. Multiple AAV serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ) enable AAVs to efficiently infect with broad specificity.

Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications in mammalian cells. There is no question that the adenovirus is the most effective means of delivering genes in vivo and in vitro. However construction of adenovirus vectors and virus packaging is time-consuming and labor intensive.

Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro). Though a number of viruses have been developed, interest has centered on four types: retrovirus, lentivirus, adenovirus and adeno-associated virus (AAV).

Protein production is a biotechnological process of generating specific proteins. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. The process includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations.

A genomic library is a collection of DNA covering the entire genome of an organism. The genomic library contains all DNA sequences such as expressed genes, non-expressed genes, exons and introns, promoter and terminator regions, intervening DNA sequences etc. It is commonly used for genome mapping, sequencing, particular gene’s studying and so on. As a leading supplier in genomic library construction services, Creative Biogene can provide the most affordable genomic library construction services with fastest turnaround time to satisfy your downstream needs.

A mutant library is a collection of protein variants which is becoming more and more popular in identification of vital residues controlling physiological activities and the investigation of protein optimization. Creative Biogene which possesses high-throughput gene synthesis and mutagenesis platform and highly experienced staffs is able to provide the most affordable mutant library construction services with fastest turnaround time to satisfy your downstream needs.

Methyltransferases are enzymes which transfer a methyl group from SAM to their substrates. DNA methyltransferases modify DNA by transferring a methyl group to cytosines. DNA methylation process suppresses gene expression, which is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. In addition, DNA methylation plays a key role in the development of nearly all types of cancer.

Yeast expression system is the most economical eukaryotic expression system for both secreted and intracellular proteins. Yeast expression system possesses the advantages of both prokaryotic and eukaryotic expression systems, such as high expression level, low cost, easy to scale up and proper protein folding which make it suitable for both small and large scale production of interested proteins.

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. The engineered CRISPR/Cas9 technology consists of a single guide RNA (sgRNA) and a human codon-optimized Cas9 nuclease. The sgRNA recognizes the genomic target via standard Watson–crick base pairing and acts as a scaffold for binding of the endonuclease Cas9.

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria.

According to the upstream regulator of the reporter gene, reporter cell lines can be further divided into two groups: 1) Constitutive Reporter Cell Lines in which reporters are constitutively expressed under the control of a constitutive, strong promoter such as CMV promoter; and 2) Inducible Reporter Cell Lines in which reporters are inducibly expressed under the control of a minimal promoter fused to multiple inducer response elements.

Creative Biogene provides the capacity of performing HTS-compatible large scale binding assay screening for all the GPCRs with the most competitive price. We offer a wide range of platforms to perform compounds binding assays with GPCR target. Moreover, Creative Biogene has developed robust, reliable and stable methods to help you profile your lead compounds interaction with GPCR target.