Biosafety testing is a series of assays to evaluate the potential biological risks of new biological products, including viral testing, microbial testing, cell and molecular testing, and analytic testing. Health authorities such as the European Medicines Agency (EMA) and the US Food and Drug Administration (FDA) require all biopharmaceutical products and vaccines to undergo stringent testing to ensure the product is safe and free of contaminants.

Next generation sequencing (NGS), a massively parallel or deep sequencing technique, has revolutionized genomic research. Compare with the first generation sequencing, the next generation sequencing is characterized by high accuracy, fast speed and low cost. So far, there are many NGS platforms involving different sequencing technologies.

Biopharmaceutical products, such as therapeutic proteins and vaccines, are produced by fermentation using either bacterial or eukaryotic cells. The cells used to produce biopharmaceuticals can be sources of a range of complex, heterogeneous, and potentially unsafe impurities, and host cell DNA is among these. The residual host cell DNA may result in tumors or adverse reactions.

Viral testing and clearance is an essential quality control procedure in manufacture process of drug and clinic products. Virus contamination is a terrible problem for which may cause bad effects on body, even severe illness. In order to eliminate this hazard, doing rigorous viral test and clearance is a nice choice.

Creative Biogene has established a number of mammalian cell lines to use for protein expression with the most common being CHO (Chinese hamster ovary) and HEK 293 (Human embryonic kidney). HEK 293 cells exhibit the highest level of polyethyleneimine (PEI)-mediated transfection with 50–80% of cells showing green fluorescent protein (GFP) expression, and are widely used for the production of recombinant proteins both by transient transfection and by the formation of stable cell lines. Other cell lines such as COS and Vero, HeLa, and NS0 have also been used for structural studies.

Creative Biogene offers high quality, fast turnaround and cost-effective Stable Cell Line Generation Service for protein expression, gene knockdown or genome editing that meet your specific research needs. Based on many years' experience on cell line development, Creative Biogene's cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements.

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing.

Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA (~150 kb) virus in the family Herpesviridae. There are two HSV species: Herpes Simplex Virus Type I (HSV-1) and Herpes Simplex Virus Type II (HSV-2), both of which can infect humans and cause diseases.

Retroviral vectors are engineered to transfer and integrate target genetic material into the genomes of host cells. Retroviruses have several distinct advantages over other vectors, especially when permanent gene transfer is the preferred outcome. Some disadvantages of retroviruses are that they can only transduce dividing cells, and that they integrate somewhat arbitrarily into genome which has the potential to lead to oncogenesis.

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

According to the upstream regulator of the reporter gene, reporter cell lines can be further divided into two groups: 1) Constitutive Reporter Cell Lines in which reporters are constitutively expressed under the control of a constitutive, strong promoter such as CMV promoter; and 2) Inducible Reporter Cell Lines in which reporters are inducibly expressed under the control of a minimal promoter fused to multiple inducer response elements.

Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library, subtractive cDNA library, normalized cDNA library, full length cDNA library, yeast two-hybrid cDNA library and SSH cDNA library. Creative Biogene’s goal is to provide you with the most affordable and high-quality cDNA libraries construction service to ensure your satisfaction in a timely and professional manner.

Stable cell lines with specific gene over-expression or knock-down are very helpful in gene function analysis, target discovery, target validation, assay development, and compound screening. However, generation of stable cell lines is a time-consuming and expensive process.

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. The engineered CRISPR/Cas9 technology consists of a single guide RNA (sgRNA) and a human codon-optimized Cas9 nuclease.

Recombinant viruses are versatile and highly efficient tools commonly used by molecular biologist for gene delivery and expression in cells. During a period of development, Creative Biogene has already possessed sophisticated equipments, advanced technologies and highly experienced staffs which are available to provide you with GMP grade recombinant viruses.