Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro). Though a number of viruses have been developed, interest has centered on four types: retrovirus, lentivirus, adenovirus and adeno-associated virus (AAV).

Protein production is a biotechnological process of generating specific proteins. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. The process includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations.

A genomic library is a collection of DNA covering the entire genome of an organism. The genomic library contains all DNA sequences such as expressed genes, non-expressed genes, exons and introns, promoter and terminator regions, intervening DNA sequences etc. It is commonly used for genome mapping, sequencing, particular gene’s studying and so on. As a leading supplier in genomic library construction services, Creative Biogene can provide the most affordable genomic library construction services with fastest turnaround time to satisfy your downstream needs.

A mutant library is a collection of protein variants which is becoming more and more popular in identification of vital residues controlling physiological activities and the investigation of protein optimization. Creative Biogene which possesses high-throughput gene synthesis and mutagenesis platform and highly experienced staffs is able to provide the most affordable mutant library construction services with fastest turnaround time to satisfy your downstream needs.

Methyltransferases are enzymes which transfer a methyl group from SAM to their substrates. DNA methyltransferases modify DNA by transferring a methyl group to cytosines. DNA methylation process suppresses gene expression, which is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. In addition, DNA methylation plays a key role in the development of nearly all types of cancer.

Yeast expression system is the most economical eukaryotic expression system for both secreted and intracellular proteins. Yeast expression system possesses the advantages of both prokaryotic and eukaryotic expression systems, such as high expression level, low cost, easy to scale up and proper protein folding which make it suitable for both small and large scale production of interested proteins.

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. The engineered CRISPR/Cas9 technology consists of a single guide RNA (sgRNA) and a human codon-optimized Cas9 nuclease. The sgRNA recognizes the genomic target via standard Watson–crick base pairing and acts as a scaffold for binding of the endonuclease Cas9.

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria.

According to the upstream regulator of the reporter gene, reporter cell lines can be further divided into two groups: 1) Constitutive Reporter Cell Lines in which reporters are constitutively expressed under the control of a constitutive, strong promoter such as CMV promoter; and 2) Inducible Reporter Cell Lines in which reporters are inducibly expressed under the control of a minimal promoter fused to multiple inducer response elements.

Creative Biogene provides the capacity of performing HTS-compatible large scale binding assay screening for all the GPCRs with the most competitive price. We offer a wide range of platforms to perform compounds binding assays with GPCR target. Moreover, Creative Biogene has developed robust, reliable and stable methods to help you profile your lead compounds interaction with GPCR target.