According to the upstream regulator of the reporter gene, reporter cell lines can be further divided into two groups: 1) Constitutive Reporter Cell Lines in which reporters are constitutively expressed under the control of a constitutive, strong promoter such as CMV promoter; and 2) Inducible Reporter Cell Lines in which reporters are inducibly expressed under the control of a minimal promoter fused to multiple inducer response elements.

Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library, subtractive cDNA library, normalized cDNA library, full length cDNA library, yeast two-hybrid cDNA library and SSH cDNA library. Creative Biogene’s goal is to provide you with the most affordable and high-quality cDNA libraries construction service to ensure your satisfaction in a timely and professional manner.

Stable cell lines with specific gene over-expression or knock-down are very helpful in gene function analysis, target discovery, target validation, assay development, and compound screening. However, generation of stable cell lines is a time-consuming and expensive process.

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. The engineered CRISPR/Cas9 technology consists of a single guide RNA (sgRNA) and a human codon-optimized Cas9 nuclease.

Recombinant viruses are versatile and highly efficient tools commonly used by molecular biologist for gene delivery and expression in cells. During a period of development, Creative Biogene has already possessed sophisticated equipments, advanced technologies and highly experienced staffs which are available to provide you with GMP grade recombinant viruses.

Stable cell lines with specific gene over-expression or knock-down are very helpful in gene function analysis, target discovery, target validation, assay development, and compound screening. However, generation of stable cell lines is a time-consuming and expensive process.

Protein phosphorylation is vital for normal cellular function and a major control mechanism of a wide range of physiological processes. Phosphate groups are post-translational modifications that regulate many disease states, including cancers, neurological diseases, diabetes, pain, autoimmune disorders, and cardiovascular diseases.

Mutagenesis in the laboratory is an important molecular biology technique that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products. It is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, or for protein engineering. With years of experience in mutagenesis field, Creative Biogene can offer the most affordable and high-quality gene mutagenesis service to meet your various needs.

miRNAs play important roles in various cellular processes including apoptosis, cell division, and proliferation and others. Investigations of miRNAs will help to decipher protein functions and even discover new drugs. Creative Biogene is committed to providing outstanding miRNA products and comprehensive services to facilitate and simplify miRNA investigation.

Ubiquitin modifications can be reversed by the reaction of enzymes collectively known as deubiquitinating enzymes (DUBs). Deubiquitination is performed by the action of DUBs, ubiquitin specific proteases that can reverse target-protein ubiquitination by editing or disassembling polyubiquitin chains. DUBs play an important role in several aspects of the ubiquitin-proteasome system, and mutations in genes expressing DUBs have been implicated in a number of diseases, including hereditary cancer and neurodegeneration.

Transporters are a kind of membrane proteins controlling uptake and efflux of essential nutrients ions, neurotransmitters, cellular waste, environmental toxins, and drugs, into and out of cells and organelles. Therefore, transporters play a significant important role in drug's clinical efficacy and safety. Currently, transporters are used as therapeutic targets to treat numerous diseases such as diabetes, asthma, cardiovascular diseases, major depression, neuroscience, hypertension and constipation.

Adeno-associated virus (AAV) has been widely used by scientists to deliver exogenous genetic material into cultured cells or live animal models. AAV can infect both dividing and non-dividing cells. Multiple AAV serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ) enable AAVs to efficiently infect with broad specificity.

Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications in mammalian cells. There is no question that the adenovirus is the most effective means of delivering genes in vivo and in vitro. However construction of adenovirus vectors and virus packaging is time-consuming and labor intensive.

Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro). Though a number of viruses have been developed, interest has centered on four types: retrovirus, lentivirus, adenovirus and adeno-associated virus (AAV).

Protein production is a biotechnological process of generating specific proteins. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. The process includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations.

A genomic library is a collection of DNA covering the entire genome of an organism. The genomic library contains all DNA sequences such as expressed genes, non-expressed genes, exons and introns, promoter and terminator regions, intervening DNA sequences etc. It is commonly used for genome mapping, sequencing, particular gene’s studying and so on. As a leading supplier in genomic library construction services, Creative Biogene can provide the most affordable genomic library construction services with fastest turnaround time to satisfy your downstream needs.

A mutant library is a collection of protein variants which is becoming more and more popular in identification of vital residues controlling physiological activities and the investigation of protein optimization. Creative Biogene which possesses high-throughput gene synthesis and mutagenesis platform and highly experienced staffs is able to provide the most affordable mutant library construction services with fastest turnaround time to satisfy your downstream needs.